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Some extracellular-flux protocols add L-carnitine when testing long-chain fatty-acid-supported respiration.A published Seahorse XF protocol for neonatal mouse cardiomyocytes (P0P1, plated at 25,000 cells/well) used a 50 mM L-carnitine stock (stored at 20 C, stable for 612 months), diluted to a final working concentration of 0.5 mM in FAO assay medium containing palmitate-BSA (1 mM palmitate / 0.2 mM fatty-acid-free BSA) as the substrate

Note that due to the relative small size of the rats, rat fistulas regularly appeared as large and complex, and thereby, in this respect, corresponded to the worst presentation in the patients (Klicek et al., 2008

Cook over medium to high heat turning with tongs * occasionally until it is crisped

Veiligheid en kwaliteitscontrole De kwaliteit van het peptide is bepalend voor effectiviteit en veiligheid